T21E3.1 | EGG sterile (unfertilizable) 4

No description available.

Graphics for egg-4

3'UTR Zoom

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Legend: Blue:ORF, Gray:Updated 3'UTRome V3 dataset.

3'UTR mapped for egg-4

If available, we display here the 3'UTRs sequences we obtained by our analysis (in FASTA format). Putative canonical PAS sites, if found, are highlighted in yellow.

1 ID: 20164 - Tier: 1 - Name: egg-4 - Cosmid: T21E3.1 - WBGeneID: WBGene00020652 - Length: 100 - PAS: tataaa
Cluster Coverage (%): 2445 reads (100.00%)
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id Name Chr Strand Start End Length PAS Coverage
20164 egg-4 I + 3934715 3934814 100nt tataaa (-23nt) 2445 reads

This 3'UTR isoform has been detected in the following tissues:
UTRome v31 Intestine2 Pharynx2 Body Muscle2 Arcade cells2 GABA neurons2 NMDA neurons2 Hypodermis2 Seam cells2
1Murari et al., 2023
2Blazie et al., 2016 - Alternative polyadenylation directs tissue specific miRNA targeting in Caenorhabditis elegans somatic tissues. - under review (mixed stages & tissue-specific datasets)
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Updated miRanda Targets for egg-4 (Murari et al., submitted)

egg-4 transcript has been predicted to be targeted by the following miRNAs:

ID miRNA Target Gene Score Energy % Binding (Target) % Binding (miRNA)
34401 cel-miR-232-3p egg-4 166 -15.99 75.00 85.00
77013 cel-miR-1819-5p egg-4 143 -9.37 68.42 73.68
82375 cel-miR-1830-5p egg-4 141 -8.50 75.00 75.00

Predicted or Experimental Interactors for egg-4 (WormBase)

egg-4 has been predicted to interact with the following genes (data from WS200):

  • car-1 Lee I et al. (2008)
  • chs-1 Lee I et al. (2008)
    chs-1 encodes a chitin synthase, paralogous to CHS-2; chs-1 is required for embryonic chitin synthesis, eggshell synthesis and osmotic integrity, error-free chromosomal segregation during meiosis, polar body extrusion, association of the sperm pronucleus/centrosome complex (SPCC) with the early embryonic cortex, localization of PAR-2 in early embryos, posterior localization of P granules or PIE-1, and pseudocleavage; CHS-1 is also, independently of chitin synthesis, required for normal localization of MBK-2 to cortex and punctae in early embryos; CHS-1 is dispensable for synthesis of pharyngeal cuticle, since chs-1(ok1120) mutants have it normally; CHS-1 is predicted to be in the plasma membrane rather than the Golgi apparatus.
  • cyb-2.1 Lee I et al. (2008)
    none available
  • fbxa-215 Lee I et al. (2008)
  • gld-1 Lee I et al. (2008)
    gld-1 encodes a protein containing a K homology RNA binding domain that is required for meiotic cell cycle progression during oogenesis in parallel with gld-2, and also affects spermatogenesis; it physically interacts with its putative mRNA targets in vitro and with FOG-2, and is expressed in the cytoplasm at high levels during meiotic prophase.
  • glh-1 Lee I et al. (2008)
    glh-1 encodes a putative DEAD-box RNA helicase that contains four CCHC zinc fingers and is homologous to Drosophila VASA, a germ-line-specific, ATP-dependent RNA helicase; at permissive temperature, GLH-1 is required redundantly with GLH-4 for proper germ-line development and fertility, specifically for regulating the normal extent of germ-line proliferation, oogenesis, and the production of functional sperm; GLH-1 activity is also likely required for the wild-type morphology of P granules and for localization of several protein components, such as PGL-1, but not for accumulation of P granule mRNAs; GLH-1 interacts in vivo with CSN-5, a COP9 signalosome component, and in vitro with itself and with KGB-1, a JNK-like MAP kinase, ZYX-1, a LIM domain-containing zyxin homologue, and GLH-3; GLH-1 is a constitutive P granule component and thus, with the exception of mature sperm, is expressed in germ cells at all stages of development; consistent with its P granule localization, GLH-1 is cytoplasmic in oocytes and the early embryo, while perinuclear in all later developmental stages as well as in the distal and medial regions of the hermaphrodite gonad; GLH-1 is also expressed in males.
  • gna-2 Lee I et al. (2008)
  • him-3 Lee I et al. (2008)
    him-3 encodes a homolog of the S. cerevisiae HOP1 protein which is required for the formation of the synaptonemal complex during meiosis; him-3 is required for synapsis and chiasma formation during meiotic recombination and for chromosome segregation; him-3 mutants show a high frequency of males in the population as well as a high frequency of arrested embryos due to defects in X-chromosome segregation; HIM-3 localizes to the meiotic chromosome associating with both unsynapsed and synapsed chromosomes.
  • hke-4.1 Lee I et al. (2008)
    none available
  • hmg-12 Lee I et al. (2008)
    none available


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