coq-4
T03F1.2 | COenzyme Q (ubiquinone) biosynthesis 4
coq-4 encodes an ortholog of S. cerevisiae COQ4; while COQ-4 is conserved between eukaryotes and bacteria, its biochemical function is unknown; by orthology, COQ-4 is predicted to peripherally associate with the matrix face of the mitochondrial inner membrane, in a complex with COQ-3 (and perhaps COQ-6), and to be required to maintain a steady-state level of CLK-1/COQ-7 protein; COQ-4 is required for ubiquinone (coenzyme Q9) biosynthesis and for normally short lifespan; coq-4(RNAi) animals have reduced levels of coenzyme Q9 and superoxide, and have abnormally long lifespans.
Graphics for coq-4
3'UTR Zoom
Locus
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Operon Information
This gene is part of the operon CEOP1124 (I:3870066..3867543)CEOP1124 contains 2 genes in the following order:
Blumenthal et al., Nature 417, 851-854
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3'UTR mapped for coq-4
If available, we display here the 3'UTRs sequences we obtained by our analysis (in FASTA format). Putative canonical PAS sites, if found, are highlighted in yellow.1
ID: 762 -
Tier: 1 -
Name: coq-4 -
Cosmid: T03F1.2 -
WBGeneID: WBGene00000764 -
Length: 48 -
PAS: aatgaa
Cluster Coverage (%): 357 reads (38.30%)
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id | Name | Chr | Strand | Start | End | Length | PAS | Coverage |
---|---|---|---|---|---|---|---|---|
762 | coq-4 | I | + | 3870064 | 3870111 | 48nt | aatgaa (-8nt) | 357 reads |
This 3'UTR isoform has been detected in the following tissues:
UTRome v31 | Intestine2 | Pharynx2 | Body Muscle2 | Arcade cells2 | GABA neurons2 | NMDA neurons2 | Hypodermis2 | Seam cells2 |
---|---|---|---|---|---|---|---|---|
  |
2Blazie et al., 2016 - Alternative polyadenylation directs tissue specific miRNA targeting in Caenorhabditis elegans somatic tissues. - under review (mixed stages & tissue-specific datasets)
>|3'UTR|48nt|I:3870064..3870111|PAS:aatgaa
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2
ID: 763 -
Tier: 1 -
Name: coq-4 -
Cosmid: T03F1.2 -
WBGeneID: WBGene00000764 -
Length: 63 -
PAS: aattaa
Cluster Coverage (%): 575 reads (61.70%)
(See this 3'UTR in GBrowse!) (See this Gene in GBrowse!)
id | Name | Chr | Strand | Start | End | Length | PAS | Coverage |
---|---|---|---|---|---|---|---|---|
763 | coq-4 | I | + | 3870064 | 3870126 | 63nt | aattaa (-20nt) | 575 reads |
This 3'UTR isoform has been detected in the following tissues:
UTRome v31 | Intestine2 | Pharynx2 | Body Muscle2 | Arcade cells2 | GABA neurons2 | NMDA neurons2 | Hypodermis2 | Seam cells2 |
---|---|---|---|---|---|---|---|---|
  |
2Blazie et al., 2016 - Alternative polyadenylation directs tissue specific miRNA targeting in Caenorhabditis elegans somatic tissues. - under review (mixed stages & tissue-specific datasets)
>|3'UTR|63nt|I:3870064..3870126|PAS:aattaa
uauagauuugaucuugaauuucucagugauaaugaaaaaaugaauuaauuaauuuuuuugga
Updated miRanda Targets for coq-4 (Murari et al., submitted)
coq-4 transcript has been predicted to be targeted by the following miRNAs:ID | miRNA | Target Gene | Score | Energy | % Binding (Target) | % Binding (miRNA) |
---|---|---|---|---|---|---|
70853 | cel-miR-797-3p | coq-4 | 162 | -16.35 | 73.68 | 84.21 | 121027 | cel-miR-5546-3p | coq-4 | 141 | -4.63 | 87.50 | 100.00 |
Predicted or Experimental Interactors for coq-4 (WormBase)
coq-4 has been predicted to interact with the following genes (data from WS200):- alh-4 Lee I et al. (2008)
none available - clk-1 Lee I et al. (2008)
clk-1 encodes the C. elegans ortholog of COQ7/CAT5, a highly conserved demethoxyubiquinone (DMQ) hydroxylase that is necessary for the biosynthesis of ubiquinone (coenzyme Q, Q9) from 5-demethoxyubiquinone (DMQ9); in C. elegans, CLK-1 activity is required for normal physiological rates of growth, development, behavior, and aging, as well as for normal brood sizes. - coq-3 Lee I et al. (2008)
coq-3 encodes a putative 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase, orthologous to S. cerevisiae COQ3; by orthology, COQ-3 is predicted to be in a complex with COQ-4 (and perhaps COQ-6), and to be required for two different steps of ubiquinone synthesis; COQ-3 is required for viability, ubiquinone (coenzyme Q9) biosynthesis and for normally short lifespan; coq-3 null mutations are lethal; coq-3(RNAi) animals have reduced levels of coenzyme Q9 and superoxide, and have abnormally long lifespans. - coq-5 Lee I et al. (2008)
coq-5 encodes a putative 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, homologous to COQ5 in S. cerevisiae and to a family of methyltransferases involved in ubiquinone, menaquinone, biotin and sterol biosynthesis and in phosphatidylethanolamine methylation; COQ-5 is required for ubiquinone (coenzyme Q9) biosynthesis and for normally short lifespan; coq-5 can transgenically rescue a coq-5 deletion in S. cerevisiae; coq-5(RNAi) animals have reduced levels of coenzyme Q9 and superoxide, and have abnormally long lifespans. - dcs-1 Lee I et al. (2008)
dcs-1 encodes a scavenger mRNA decapping enzyme; dcs-1 exhibits hydrolase activity in vitro, with specificity for 7meGMP in its primary nucleoside monophosphate binding site; dcs-1 is the upstream gene in an operon with fre-1, which encodes an NADPH-dependent flavin reductase; reporter fusions using dcs-1 upstream sequence direct expression throughout the life cycle in neurons and pharyngeal muscle; expression is present in dauer larvae and enhanced by heat shock. - kin-1 Lee I et al. (2008)
kin-1 encodes multiple isoforms of a serine/threonine protein kinase that is orthologous to cAMP-dependent protein kinase (protein kinase A or PKA) catalytic subunits; KIN-1 is able to phosphorylate histone H2B in vitro and is associated with both particulate (~65%) and cytosolic (35%) cellular fractions; KIN-1 is expressed at low levels during embryogenesis, but its expression level increases in late embryogenesis, whereupon KIN-1 expression remains relatively high throughout larval and adult development; kin-1 mRNA levels, however, are fairly constant throughout development, suggesting that KIN-1 expression is regulated post-transcriptionally; as loss of kin-1 activity via large-scale RNAi screens results in no obvious defects, the precise role of kin-1 in C. elegans development and/or behavior is not yet known. - pas-3 Lee I et al. (2008)
pas-3 encodes that affects embryonic viability, growth, fertility, and locomotion. - pas-7 Lee I et al. (2008)
pas-7 encodes a proteasome alpha-type three subunit of the core 20S proteasome subcomplex; loss of pas-7 activity via RNAi results in several defects including embryonic and larval lethality, sterility, and abnormal meiotic progression. - pnc-1 Lee I et al. (2008)
pnc-1 encodes, by alternative splicing, two isoforms of a putative pyrazinamidase/nicotinamidase, orthologous to budding yeast Pnc1p, that is required for normal lifespan; PNC-1 is paralogous to Y57G11C.47; because its overexpression increases survival during oxidative stress, PNC-1 may be required for oxidative resistance in vivo; pnc-1(RNAi) animals are abnormally short-lived. - prdx-6 Lee I et al. (2008)
none available
More on coq-4
Related Papers
There are no recent papers related to coq-4.Related coq Genes
The APAome Project is currently funded by the School of Life Sciences at Arizona State University